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(A) SK-Mel-28 or (B) MCF-7 cells were incubated for 24 h or 48 h/37°C with either control medium, or this medium 45 containing 311 (25 μM), DFO (100 μM), or FAC (100 μg/mL). Total protein was then extracted and immunoblotting for TfR1 (positive control for iron-depletion), <t>SAT1,</t> PAOX, SMOX and β-actin was performed. Results are typical blots from 3 experiments. The quantitation represents mean ± SD (3 independent experiments). Relative to the respective control: *p < 0.05, **p < 0.01, ***p < 0.001.
Sat1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sat1+antibody+-+bsa+free/SAT1+Antibody+-+BSA+Free/pmc06487307-108-76-82
Average 92 stars, based on 1 article reviews
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(A) SK-Mel-28 or (B) MCF-7 cells were incubated for 24 h or 48 h/37°C with either control medium, or this medium 45 containing 311 (25 μM), DFO (100 μM), or FAC (100 μg/mL). Total protein was then extracted and immunoblotting for TfR1 (positive control for iron-depletion), SAT1, PAOX, SMOX and β-actin was performed. Results are typical blots from 3 experiments. The quantitation represents mean ± SD (3 independent experiments). Relative to the respective control: *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: COUPLING OF THE POLYAMINE AND IRON METABOLISM PATHWAYS IN THE REGULATION OF PROLIFERATION: MECHANISTIC LINKS TO ALTERATIONS IN KEY POLYAMINE BIOSYNTHETIC AND CATABOLIC ENZYMES

doi: 10.1016/j.bbadis.2018.05.007

Figure Lengend Snippet: (A) SK-Mel-28 or (B) MCF-7 cells were incubated for 24 h or 48 h/37°C with either control medium, or this medium 45 containing 311 (25 μM), DFO (100 μM), or FAC (100 μg/mL). Total protein was then extracted and immunoblotting for TfR1 (positive control for iron-depletion), SAT1, PAOX, SMOX and β-actin was performed. Results are typical blots from 3 experiments. The quantitation represents mean ± SD (3 independent experiments). Relative to the respective control: *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Primary antibodies used in this study include: TfR1 (Cat. #: 136800: 1:2,000) from Life Technologies (Carlsbad, CA); AMD1 (Cat. #: PA5–31540; 1:1,000), from ThermoFisher Scientific (Waltham, MA); AZIN1 (Cat. #: ab57169; 1:500), ODC (Cat. #: ab97395; 1:1,000), Antizyme (Cat. #: ab85221; 1:100), MAT2α (Cat. #: ab77471; 1:2,000), ADI1 (Cat. #: ab37877; 1:1,000) and ADI1 (Cat. #: ab154689; 1:1,000) from Abcam (Cambridge, UK); PAOX (Cat. #: LS- {"type":"entrez-nucleotide","attrs":{"text":"B12847","term_id":"2093969"}} B12847 ; 1:1,000) from LifeSpan Biosciences, Inc. (Seattle, WA); SAT1 (Cat. #: NB110–41622; 1:1,000) from Novus Biologicals (Littleton, CO); SMOX (Cat. #: 15052–1-AP; 1:1,000) from Proteintech (Rosemont, IL); c-Myc (Cat. #: 5605; 1:1,000), p53 (Cat. #: 48818; 1:1,000) from Cell Signaling Technology (Danvers, MA); SLC22A16 (Cat. #: HPA036902; 1:1,000), SLC3A2 (Cat. #: HPA017980; 1:1,000), SMS (Cat. #: HPA029849, 1:1,000), SRM (Cat. #: HPA029528; 1:1,000) and β-actin (Cat. #: A5316; 1:5,000) were from Sigma-Aldrich (St. Louis, MO).

Techniques: Incubation, Control, Western Blot, Positive Control, Quantitation Assay

Cells were transiently transfected for 48 h/37°C with esiRNA pools specific for p53, or non-targeting pool (NTP) control siRNA (siNC*), all at a final concentration of 20 nM. The cells were then incubated with control medium, or this medium containing 311 (25 μM), DFO (100 μM), or FAC (100 μg/mL) for 48 h/37°C. Total protein was then extracted and expression of TfR1, p53, ADI, ODC, Antizyme, AZIN1, SAT1 and β-actin were then assessed by immunoblot analysis. Results are typical blots from 3 experiments. The quantitation represents mean ± SD (3 independent experiments). Relative to the respective control: *p < 0.05, **p < 0.01, ***p < 0.001; Relative to the respective siNC* condition: # p < 0.05; ## p < 0.01; ### p < 0.001.

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: COUPLING OF THE POLYAMINE AND IRON METABOLISM PATHWAYS IN THE REGULATION OF PROLIFERATION: MECHANISTIC LINKS TO ALTERATIONS IN KEY POLYAMINE BIOSYNTHETIC AND CATABOLIC ENZYMES

doi: 10.1016/j.bbadis.2018.05.007

Figure Lengend Snippet: Cells were transiently transfected for 48 h/37°C with esiRNA pools specific for p53, or non-targeting pool (NTP) control siRNA (siNC*), all at a final concentration of 20 nM. The cells were then incubated with control medium, or this medium containing 311 (25 μM), DFO (100 μM), or FAC (100 μg/mL) for 48 h/37°C. Total protein was then extracted and expression of TfR1, p53, ADI, ODC, Antizyme, AZIN1, SAT1 and β-actin were then assessed by immunoblot analysis. Results are typical blots from 3 experiments. The quantitation represents mean ± SD (3 independent experiments). Relative to the respective control: *p < 0.05, **p < 0.01, ***p < 0.001; Relative to the respective siNC* condition: # p < 0.05; ## p < 0.01; ### p < 0.001.

Article Snippet: Primary antibodies used in this study include: TfR1 (Cat. #: 136800: 1:2,000) from Life Technologies (Carlsbad, CA); AMD1 (Cat. #: PA5–31540; 1:1,000), from ThermoFisher Scientific (Waltham, MA); AZIN1 (Cat. #: ab57169; 1:500), ODC (Cat. #: ab97395; 1:1,000), Antizyme (Cat. #: ab85221; 1:100), MAT2α (Cat. #: ab77471; 1:2,000), ADI1 (Cat. #: ab37877; 1:1,000) and ADI1 (Cat. #: ab154689; 1:1,000) from Abcam (Cambridge, UK); PAOX (Cat. #: LS- {"type":"entrez-nucleotide","attrs":{"text":"B12847","term_id":"2093969"}} B12847 ; 1:1,000) from LifeSpan Biosciences, Inc. (Seattle, WA); SAT1 (Cat. #: NB110–41622; 1:1,000) from Novus Biologicals (Littleton, CO); SMOX (Cat. #: 15052–1-AP; 1:1,000) from Proteintech (Rosemont, IL); c-Myc (Cat. #: 5605; 1:1,000), p53 (Cat. #: 48818; 1:1,000) from Cell Signaling Technology (Danvers, MA); SLC22A16 (Cat. #: HPA036902; 1:1,000), SLC3A2 (Cat. #: HPA017980; 1:1,000), SMS (Cat. #: HPA029849, 1:1,000), SRM (Cat. #: HPA029528; 1:1,000) and β-actin (Cat. #: A5316; 1:5,000) were from Sigma-Aldrich (St. Louis, MO).

Techniques: Transfection, esiRNA, Control, Concentration Assay, Incubation, Expressing, Western Blot, Quantitation Assay